minimal her1 and her2 expressions in cho and hek-293 cells cause them appropriate negative cells for hers-related studies
نویسندگان
چکیده
background: understanding of cellular and molecular mechanisms involved in cell/tumor growth and progression has led to molecular-targeted therapy. her1 and her2 are two main oncogenic components of her-receptor family considered as targets for cancer research and therapy. reliable results of her- related research and targeted therapies dependent upon cell line with known her expression status. but there are no published studies focusing on determination of her1 and her2 receptors expression in cho and hek-293 cell lines. materials and methods: absolute expressions of her1 and her2 in cho, hek-293, mda-mb-468 and skbr3 cells were evaluated using quantitative real-time pcr. absolute expression quantification was carried out by serial dilution plasmids harboring her1 and her2 to extrapolate standard curve. results: real-time pcr amplification was successfully optimized by her1 and her2 plasmids with high efficiency and their copy numbers were calculated. the results showed that her1 was not expressed in cho and hek-293 cells but these cells express her2 up to 9.4 × 102 and 1.1 × 105 copy/µg, respectively. mda-mb-468 and skbr3 cells express her1 up to 8.4 × 105 and 9.7 × 104, respectively. in addition, the expression of her2 is higher on the surface of skbr3 cell (6.5 × 106 copy/µg). conclusion: our study provides a proven insight to her1 and her2 expression status in cho, hek-293, mda-mb-468 and skbr3 cells. heterologous expression of her1 and her2 in cell lines is the first step in structural and functional studies. since her1 is not expressed in cho and hek-293 endogenously, these cells are ideal for her receptor related studies.
منابع مشابه
Minimal HER1 and HER2 expressions in CHO and HEK-293 cells cause them appropriate negative cells for HERs-related studies
Background: Understanding of cellular and molecular mechanisms involved in cell/tumor growth and progression has led to molecular-targeted therapy. HER1 and HER2 are two main oncogenic components of HER-receptor family considered as targets for cancer research and therapy. Reliable results of HER- related research and targeted therapies dependent upon cell line with known HER expression status....
متن کاملInfluence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells
Background Glutamine is an essential component in culture media for most of the mammalian cell lines. It is often used as an alternative source of energy by cells, along with glucose. Glutamine metabolism induces ammonia accumulation in cell culture. Elevated ammonia concentration above 2 mM has been shown to have negative impact on both cell growth and recombinant protein productivity [1-4]. I...
متن کاملDifferences in the production of hyperglycosylated IFN alpha in CHO and HEK 293 cells
Background IFN alpha is an important cytokine of the immune system. It has the ability to interfere with virus replication exerting antiviral activity. Moreover, it displays antiproliferative activity and can profoundly modulate the immune response. IFN4N (or hyperglycosylated IFN alpha) is an IFN-alpha2b mutein developed in our laboratory using glycoengineering strategies. This molecule contai...
متن کاملMyricetin inhibits Kv 1 . 5 channels in HEK 293 cells
Myricetin (Myr) is a f lavonoid that exerts anti-arrhythmic effects. However, its potential effects on ion channels have remained elusive. The aim of the present study was to investigate the effects of Myr on Kv1.5 channels in HEK293 cells. The current of Kv1.5 channels (Ikur) in HEK293 cells was recorded using the whole-cell patch-clamp technique and the expression of the Kv1.5 protein was mea...
متن کاملCharacterization of a Mutant Dopamine Transporter in HEK-293 Cells
................................................. 3 Background.............................................. 4 Materials and Methods...................................8 Results....................................................12 Discussion................................................14 Acknowledgments.......................................16 References.........................................
متن کاملsimulation and experimental studies for prediction mineral scale formation in oil field during mixing of injection and formation water
abstract: mineral scaling in oil and gas production equipment is one of the most important problem that occurs while water injection and it has been recognized to be a major operational problem. the incompatibility between injected and formation waters may result in inorganic scale precipitation in the equipment and reservoir and then reduction of oil production rate and water injection rate. ...
منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
research in molecular medicineجلد ۱، شماره ۳، صفحات ۶-۱۲
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023